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saponin based permeabilization buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher saponin based permeabilization buffer
    Saponin Based Permeabilization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/saponin+permeabilization+buffer/Saponin/10__3389_slash_fimmu__2026__1816695-55-11-14
    Average 97 stars, based on 1 article reviews
    saponin based permeabilization buffer - by Bioz Stars, 2026-09
    97/100 stars

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    Incubation:

    Article Title: Combating yellow fever virus with 7-deaza-7-fluoro-2′-C-methyladenosine
    Article Snippet: Virus was incubated with cells for 2 h, then removed, and cells were overlayed with 1% methylcellulose for 48 h. For all experiments, methylcellulose was aspirated, and cells were fixed in 4% paraformaldehyde at room temperature for 20 min. For YFV-17D ELIspots, fixed cells were blocked (Pierce Clear Milk Blocking Buffer; Thermo Fisher, USA), incubated overnight with a pan-flavivirus group antigen primary antibody (anti-flavivirus C1:D1-4G2-4-15 Mouse IgG 2a; Absolute Antibody, Centennial, Colorado), then treated with secondary goat anti-mouse HRP antibody (Invitrogen) for 1 h, followed by detection of viral antigen with KPL TrueBlue Peroxidase Substrate (SeraCare, Milford, MA, USA) and imaging at 4× on a CTL plate reader. .. For YFV-DakH1279 ELIspots, cells were incubated with primary antibodies bt-3A8 (kind gift from Dr. Slifka [ ]) and 2D12 (Millipore, Sigma) in 0.1% saponin permeabilization buffer containing 2% FBS, followed by a secondary antibody (goat α-ms IgG-HRP; Invitrogen) each for 1 h at room temperature with washing (saponin buffer) in between. .. The HRP secondary was detected with 1-Step Ultra TMB-Blotting Solution (Thermo Scientific).

    Article Title: Ionizing radiation expands a p90RSK-activated patrolling monocyte subset: modulation by colchicine
    Article Snippet: After washing two times with MaxPar PBS, the cells were resuspended in MaxPar Cell Staining Buffer (Fluidigm #201068) and were blocked by Human TruStain FcX (BioLegend #422302), followed by cell surface staining for cell type identification. .. After the wash with MaxPar PBS, the cells were chilled on ice and incubated at 4 °C with 1× saponin permeabilization buffer (eBiosciences #00-8333-56), followed by two washes with MaxPar PBS. ..

    Staining:

    Article Title: Galectin-9 regulates the threshold of B cell activation and autoimmunity
    Article Snippet: Cells were fixed in 2% paraformaldehyde, stained with PE-conjugated streptavidin (BioLegend) to occupy mono-biotinylated lysozyme on the cell surface. .. Cells were then permeabilized in 0.1% Saponin permeabilization buffer (0.1% saponin, 3% FBS, 0.1% sodium azide, in PBS) and stained with Alexa Fluor-633-conjugated streptavidin (Invitrogen) to detect intracellular lysozyme by flow cytometry. .. For experiments using OP9 stromal cells: OP9 cells were labeled with 5 μM CFSE (Invitrogen).

    Article Title: Fluoxetine promotes immunometabolic defenses to mediate host-pathogen cooperation during sepsis
    Article Snippet: .. The following day cells were washed with 1xPBS and stained for 30 minutes with intracellular stains in 50 μL saponin permeabilization buffer (Invitrogen). ..

    Article Title: Fluoxetine promotes IL-10–dependent metabolic defenses to protect from sepsis-induced lethality
    Article Snippet: .. The following day, cells were washed with 1x PBS and stained for 30 min with intracellular stains in 50 μl of saponin permeabilization buffer (Invitrogen). ..

    Article Title: Galectin-9 regulates the threshold of B cell activation and autoimmunity
    Article Snippet: Cells were fixed in 2% paraformaldehyde, stained with PE-conjugated streptavidin (BioLegend) to occupy mono-biotinylated lysozyme on the cell surface. .. Cells were then permeabilized in 0.1% Saponin permeabilization buffer (0.1% saponin, 3% FBS, 0.1% sodium azide, in PBS) and stained with Alexa Fluor-633-conjugated streptavidin (Invitrogen) to detect intracellular lysozyme by flow cytometry. .. For experiments using OP9 stromal cells: OP9 cells were labeled with 5 mM CFSE (Invitrogen).

    Article Title: Fluoxetine promotes IL-10-dependent metabolic defenses to protect from sepsis-induced lethality.
    Article Snippet: .. The following day, cells were washed with 1x PBS and stained for 30 min with intracellular stains in 50 μl of saponin permeabilization buffer (Invitrogen). ..

    Flow Cytometry:

    Article Title: Galectin-9 regulates the threshold of B cell activation and autoimmunity
    Article Snippet: Cells were fixed in 2% paraformaldehyde, stained with PE-conjugated streptavidin (BioLegend) to occupy mono-biotinylated lysozyme on the cell surface. .. Cells were then permeabilized in 0.1% Saponin permeabilization buffer (0.1% saponin, 3% FBS, 0.1% sodium azide, in PBS) and stained with Alexa Fluor-633-conjugated streptavidin (Invitrogen) to detect intracellular lysozyme by flow cytometry. .. For experiments using OP9 stromal cells: OP9 cells were labeled with 5 μM CFSE (Invitrogen).

    Article Title: Galectin-9 regulates the threshold of B cell activation and autoimmunity
    Article Snippet: Cells were fixed in 2% paraformaldehyde, stained with PE-conjugated streptavidin (BioLegend) to occupy mono-biotinylated lysozyme on the cell surface. .. Cells were then permeabilized in 0.1% Saponin permeabilization buffer (0.1% saponin, 3% FBS, 0.1% sodium azide, in PBS) and stained with Alexa Fluor-633-conjugated streptavidin (Invitrogen) to detect intracellular lysozyme by flow cytometry. .. For experiments using OP9 stromal cells: OP9 cells were labeled with 5 mM CFSE (Invitrogen).



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