saponin based permeabilization buffer (Thermo Fisher)
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 
97
Structured Review
Thermo Fisher
saponin based permeabilization buffer
Saponin Based Permeabilization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/saponin+permeabilization+buffer/Saponin/10__3389_slash_fimmu__2026__1816695-55-11-14
Average 97 stars, based on 1 article reviews
Saponin Based Permeabilization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/saponin+permeabilization+buffer/Saponin/10__3389_slash_fimmu__2026__1816695-55-11-14
Average 97 stars, based on 1 article reviews
saponin based permeabilization buffer - by Bioz Stars,
2026-10
97/100 stars
Images
Related Articles
Incubation:Article Title: Combating yellow fever virus with 7-deaza-7-fluoro-2′-C-methyladenosine Article Snippet: Virus was incubated with cells for 2 h, then removed, and cells were overlayed with 1% methylcellulose for 48 h. For all experiments, methylcellulose was aspirated, and cells were fixed in 4% paraformaldehyde at room temperature for 20 min. For YFV-17D ELIspots, fixed cells were blocked (Pierce Clear Milk Blocking Buffer; Thermo Fisher, USA), incubated overnight with a pan-flavivirus group antigen primary antibody (anti-flavivirus C1:D1-4G2-4-15 Mouse IgG 2a; Absolute Antibody, Centennial, Colorado), then treated with secondary goat anti-mouse HRP antibody (Invitrogen) for 1 h, followed by detection of viral antigen with KPL TrueBlue Peroxidase Substrate (SeraCare, Milford, MA, USA) and imaging at 4× on a CTL plate reader. .. For YFV-DakH1279 ELIspots, cells were incubated with primary antibodies bt-3A8 (kind gift from Dr. Slifka [ ]) and 2D12 (Millipore, Sigma) in 0.1% Article Title: Ionizing radiation expands a p90RSK-activated patrolling monocyte subset: modulation by colchicine Article Snippet: After washing two times with MaxPar PBS, the cells were resuspended in MaxPar Cell Staining Buffer (Fluidigm #201068) and were blocked by Human TruStain FcX (BioLegend #422302), followed by cell surface staining for cell type identification. .. After the wash with MaxPar PBS, the cells were chilled on ice and incubated at 4 °C with 1× Staining:Article Title: Galectin-9 regulates the threshold of B cell activation and autoimmunity Article Snippet: Cells were fixed in 2% paraformaldehyde, stained with PE-conjugated streptavidin (BioLegend) to occupy mono-biotinylated lysozyme on the cell surface. .. Cells were then permeabilized in 0.1% Article Title: Fluoxetine promotes immunometabolic defenses to mediate host-pathogen cooperation during sepsis Article Snippet: .. The following day cells were washed with 1xPBS and stained for 30 minutes with intracellular stains in 50 μL Article Title: Fluoxetine promotes IL-10–dependent metabolic defenses to protect from sepsis-induced lethality Article Snippet: Cells were then washed in 1x PBS and then incubated in 25 μl of Brilliant Stain Buffer (BD Biosciences) + extracellular stains for 20 min on ice. .. The following day, cells were washed with 1x PBS and stained for 30 min with intracellular stains in 50 μl of Article Title: Galectin-9 regulates the threshold of B cell activation and autoimmunity Article Snippet: Cells were fixed in 2% paraformaldehyde, stained with PE-conjugated streptavidin (BioLegend) to occupy mono-biotinylated lysozyme on the cell surface. .. Cells were then permeabilized in 0.1% Article Title: Fluoxetine promotes IL-10-dependent metabolic defenses to protect from sepsis-induced lethality. Article Snippet: .. The following day, cells were washed with 1x PBS and stained for 30 min with intracellular stains in 50 μl of Flow Cytometry:Article Title: Galectin-9 regulates the threshold of B cell activation and autoimmunity Article Snippet: Cells were fixed in 2% paraformaldehyde, stained with PE-conjugated streptavidin (BioLegend) to occupy mono-biotinylated lysozyme on the cell surface. .. Cells were then permeabilized in 0.1% Article Title: Galectin-9 regulates the threshold of B cell activation and autoimmunity Article Snippet: Cells were fixed in 2% paraformaldehyde, stained with PE-conjugated streptavidin (BioLegend) to occupy mono-biotinylated lysozyme on the cell surface. .. Cells were then permeabilized in 0.1% |